What distinguishes acid-fast staining from Gram staining, and what color indicates an acid-fast organism after counterstain?

• A. It uses a carbol fuchsin stain with high lipid content cell walls; acid-fast organisms remain red/pink after counterstain with methylene blue or brilliant green.
• B. It uses crystal violet only; acid-fast become purple
• C. It uses safranin only; acid-fast become green
• D. It uses methylene blue primary stain; acid-fast become blue

Answer: (A)